Identification of proliferating cells in hard clams.
نویسندگان
چکیده
The origin of hemocytes, the circulating "blood cell s" of bivalve molluscs, including hard clams (Mercenaria mercenaria) has not been identified ( I, 2). Proli feration of hemocytes, however, can be recognized through their increased numbers in diseased animals. ProHferating cell nuclear amigen (PC A), or cyclin, is a protein produced during the late G I and S phases of I he cell cycle (3. 4). Using antibodies that recognize PCNA in mice, we attempted to identi fy the origin of hemocyte in the hard shell c lams. Quahog parasite unknown (QPX) is a protist that causes severe inflammation and mortality in infected c lams (5, 6). We altempted to induce hemocyte prol ife ration by exposing clams 10 QPX in a 10-1 water column in which 12 ml of undilu1ed QPX culture (at a concentration of 7* I 0 cells/ml) were added every I 0 days; by injecting QPX between the membranous mantles and Ihe right valves, 3 em ventral anterior to the siphon and into the pericardia! cavities (0.25 ml of undiluted Q PX culture); and by injecting an inert particle (India ink, 1:10 dilution in sterile seawater [7, 8]) into the pericardia! cavities. The controls consisted of two groups of clams. One group was injected with steri le eawatcr in the pericardia! cavities; the other was untreated. Groups were sampled at 24 h.
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ورودعنوان ژورنال:
- The Biological bulletin
دوره 199 2 شماره
صفحات -
تاریخ انتشار 2000